Rabbit anti‑Cat IgG Polyclonal RW-C346847-1

Antibody: Cat IgG Rabbit anti-Cat Polyclonal (Biotin) Antibody, Application: IF, ELISA, DB, ID, Reactivity: Cat, Format: Biotin, Unmodified, Other formats: Unconjugated HRP FITC, Descriptio: Cat IgG antibody RW-C346847 is a biotin-conjugated rabbit polyclonal antibody. Validated for DB, ELISA, ID and IF., Targe: Cat IgG, Synonym: Feline IgG, Hos: Rabbit, Reactivit: Cat (tested or 100% immunogen sequence identity), Clonalit: Polyclonal, Conjugation: Biotin. Also available Unconjugated or conjugated with HRP, FITC., Purificatio: Purified IgG, Modification: Unmodified, Immunoge: Purified normal IgG isolated from pooled cat serum. Freund's complete adjuvant is used in the first step of the immunization procedure., Specificit: Cat IgG, heavy and light chains., Application: Immunofluorescence
ELISA
Dot Blot
Immunodiffusion
Applications tested for the base form of this product only, Usag: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in cat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen or immune complex using a reference antibody of cat origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500. in ELISA and comparable non-precipitating antibody-binding assays between 1:1000 and 1:20000., Presentatio: Lyophilized from PBS, pH 7.2, No preservatives added, Reconstitutio: 1 ml Distilled water, Storag: Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee.

Cat: RW-52-120164-47