Rabbit anti‑Chicken Collagen V Polyclonal RW-C744075-0.5

Antibody: Collagen V Rabbit anti-Chicken Polyclonal Antibody, Application: IHC-P, IF, ELISA, RIA, Reactivity: Chicken, Format: Unconjugated, Unmodified, Descriptio: Collagen V antibody RW-C744075 is an unconjugated rabbit polyclonal antibody to chicken Collagen V. Validated for ELISA, IF, IHC and RIA., Targe: Chicken Collagen V, Hos: Rabbit, Reactivit: Chicken (tested or 100% immunogen sequence identity), Clonalit: Polyclonal, Conjugation: Unconjugated, Modification: Unmodified, Immunoge: Purified collagen type V from chicken skin, Application: IHC - Paraffin (1:200 - 1:600)
Immunofluorescence (1:40)
ELISA
Radioimmunoassay, Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 minutes at RT is recommended. Collagen type V is found as short fibrils in skin, bone and placenta. Often it is found in conjunction with other collagen types, especially type I and III. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Chicken collagen type V 100%, chicken collagen type I, II, III and X <0.1%; chicken fibronectin <0.1%; human collagen type V <0.1%., Presentatio: PBS, Reconstitutio: 0.5 ml Reconstitute in sterile distilled water., Storag: Store at -20°C., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee.

Cat: RW-52-121736-144