Rabbit anti‑Human ABCB1 / MDR1 / P Glycoprotein Polyclonal RW-A9403-50

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Antibody: ABCB1 / MDR1 / P Glycoprotein Rabbit anti-Human Polyclonal (Cytoplasmic Domain) Antibody, Application: IHC, IHC-P, Reactivity: Human, Format: Unconjugated, Unmodified, Descriptio: P Glycoprotein antibody RW-A9403 is an unconjugated rabbit polyclonal antibody to human P Glycoprotein (ABCB1 / MDR1) (Cytoplasmic Domain). Validated for IHC. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication., Targe: Human ABCB1 / MDR1 / P Glycoprotein, Synonym: ABCB1 | ABC20 | Abcb1b | CLCS | Colchicin sensitivity | CD243 | IBD13 | gp170 | MDR1 | Multidrug resistance protein 1 | P glycoprotein | P-glycoprotein 1 | P-GP | CD243 antigen | PGY1, Hos: Rabbit, Reactivit: Human (tested or 100% immunogen sequence identity), Predicte: Monkey (at least 90% immunogen sequence identity), Clonalit: Polyclonal, Conjugation: Unconjugated, Purificatio: Immunoaffinity purified, Modification: Unmodified, Immunoge: Synthetic 17 amino acid peptide from cytoplasmic domain of human ABCB1 / MDR1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%); Monkey (94%); Mouse, Hamster (82%)., Epitop: Cytoplasmic Domain, Specificit: Human ABCB1 / MDR1. BLAST analysis of the peptide immunogen showed no homology with other human proteins., Application: IHC
IHC - Paraffin (2.5 - 5 µg/ml), Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 20 ug/ml., Presentatio: PBS, 0.1% Sodium Azide, Storag: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee., About ABCB1 / MDR1 / P Glycoprotei: Uniprot: P08183 NCBI: NM_000927 NP_000918.2

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