Rabbit anti‑Arginine, dimethyl IgG Polyclonal RW-C69248-200

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Antibody: Arginine, dimethyl Rabbit Polyclonal Antibody, Application: WB, Format: Unconjugated, Unmodified, Descriptio: Arginine, dimethyl antibody RW-C69248 is an unconjugated rabbit polyclonal antibody to Arginine, dimethyl. Validated for WB., Targe: Arginine, dimethyl, Hos: Rabbit, Clonalit: IgG Polyclonal, Conjugation: Unconjugated, Purificatio: Antiserum, Modification: Unmodified, Immunoge: KLH-conjugated, synthetic peptide containing asymmetric dimethyl-arginine-glycine repeats (KGR*GR*GR*GR*GPPPPPR*GR*GR*GR*G in which R* denotes asymmetric dimethylation of arginine)., Specificit: Recognizes proteins that contain arginines that are asymmetrically dimethylated including p300, p110, p75 and Sam68. Specificity for asymmetrically dimethylated arginine was determined by ELISA with an unmethylated 'backbone' peptide or an identical peptide containing symmetrically dimethylated arginines. Species cross-reactivity: Human and mouse., Application: Western blot, Usag: Suitable for use in Western Blot. Western Blot Analysis: A 1:500-1:1000 dilution of this lot detected p110, p75, and Sam68 in RIPA lysates from Jurkat cells. Included Positive Antigen Control: Jurkat lysate. Add 2.5 ul of 2-mercaptoethanol/100 ul of lysate and boil for 5 minutes to reduce the preparation. Load 20 ug of reduced lysate per lane for minigels., Presentatio: PBS, 0.05% Sodium Azide, 30% Glycerol, Storag: Store at 4°C or -20°C. Avoid freeze-thaw cycles., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee.

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