Rabbit anti‑Human ACE2 / ACE-2 IgG Polyclonal RW-B3197-0.05

Antibody: ACE2 / ACE-2 Rabbit anti-Human Polyclonal Antibody, Application: IHC-P, IF, WB, ELISA, Reactivity: Human, Format: Unconjugated, Unmodified, Descriptio: Angiotensin-Converting Enzyme 2 (ACE2) is a transmembrane protein found in the lungs, intestines, heart, kidney, liver and arteries that plays a central role in vascular, renal, and myocardial physiology. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7. It is involved in controlling vascular function including blood pressure, and absence of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function. ACE2 is also the primary attachment receptor for HCoV-N63, SARS-CoV and SARS-CoV-2. The S1 portion of the coronavirus spike protein attaches to ACE2 presented on the host cell surface. The SARS-CoV-2 virus targets pneumocytes and macrophages expressing ACE2 in the lung, and it is believed to infect ACE2-positive cells in other tissues including the gastrointestinal tract and potentially the liver (in cholangiocytes).

Hoffmann et al., 2020, Cell. 181, 1–10. April 16, 2020, DOI: 10.1016/j.cell.2020.02.052; Zhou, P et al. Nature.579, 270–273 (2020). DOI: 10.1038/s41586-020-2012-7; Xiaoqiang Chai et al. bioRxiv 2020.02.03.931766, DOI: 10.1101/2020.02.03.931766; Tikellis, Chris, and M C Thomas. Int J Pep. vol. 2012 (2012): 256294. doi:10.1155/2012/256294; Wrap et al. Science, 13 Mar 2020:Vol. 367, Issue 6483, pp. 1260-1263. DOI: 10.1126/science.abb2507;, Targe: Human ACE2 / ACE-2, Synonym: ACE2 | ACE-related carboxypeptidase | ACEH | Metalloprotease MPROT15 | SARS-CoV2 Coronavirus Receptor | SARS-CoV-2 Coronavirus Receptor | COVID-19 viral receptor, Hos: Rabbit, Reactivit: Human (tested or 100% immunogen sequence identity), Predicte: Mouse, Rat (at least 90% immunogen sequence identity), Clonalit: IgG Polyclonal, Conjugation: Unconjugated, Purificatio: Peptide Column Affinity Chromatography, Modification: Unmodified, Immunoge: A synthetic peptide corresponding to amino acids near the center of human ACE2. The immunogen is located within aa 150-200 of ACE2., Specificit: Anti-ACE2 has no cross response to ACE1., Application: IHC - Paraffin (2.5 µg/ml)
Immunofluorescence
Western blot (0.5 - 2 µg/ml)
ELISA, Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: ACE2 antibody can be used for the detection of ACE2 by Western blot at 0.5 to 2 ug/mL. A 90 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 2 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:, Presentatio: PBS containing 0.02% Sodium Azide, Storag: Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee., About ACE2 / ACE-: Uniprot: Q9BYF1 NCBI: NM_021804 NP_068576.1

Cat: RW-52-4004-75