Rabbit anti‑Human ACPP / PAP IgG Polyclonal RW-B12704-100

Antibody: ACPP / PAP Rabbit anti-Human Polyclonal (aa215-264) Antibody, Application: IHC, IHC-P, WB, Reactivity: Human, Format: Unconjugated, Unmodified, Other formats: Biotin FITC HRP, Descriptio: PSAP (ACPP, prostatic acid phosphatase) is a dephosphorylating enzyme produced in prostatic glandular epithelium. PSAP is a positive biomarker for hyperplastic prostate in immunohistochemistry and may be used to identify prostate origin in metastatic cancers. PSAP distinguishes prostate cancer (positive) from urothelial cancer, mesonephric remnant hyperplasia and clear cell tumors (generally negative). It is also positive in bladder and rectal carcinomas. Higher expression in tumors has been correlated with increasing tumor stage, although it is thought to be a tumor suppressant by means of vitamin D-associated slowing of prostate growth. Lower expression in normal tissue is therefore thought to be a risk factor for the development of cancer. In immunohistochemistry of normal tissue, antibodies to PSAP have cytoplasmic positivity in lysosomal granules in normal prostatic epithelial and ductal cells.

References: Virchows Archiv: An International Journal of Pathology 2009-05; 454.5; 573-579, PMID: 19301031; Cancer Research 9/1/1992; 52.17; 4600-4607, PMID:1380886; The Journal of Steroid Biochemistry and Molecular Biology 2005-10; 97.43102; 37-46, PMID: 16076555; Biomolecules & Therapeutics 2013-01; 21.1; 43393, PMID: 24009853; Journal of Immunology (Baltimore, Md.: 1950) 12/15/2001; 167.12; 7150-7156, PMID: 11739538; The American Journal of Surgical Pathology 1991-08; 15.8; 785-790, PMID; 1712549; Appl Immunohistochem Mol Morphol. 2002 Sep;10(3):231-6, PMID: 12373149; Ann Diagn Pathol 2009;13:402, PMID: 19917478;, Targe: Human ACPP / PAP, Synonym: ACPP | ACP3 | 5-NT | 5-nucleotidase | Acid phosphatase, prostate | ACP-3 | Ecto-5-nucleotidase | PAP | Prostatic acid phosphatase | Thiamine monophosphatase | Prostatic acid phosphotase | TMPase, Hos: Rabbit, Reactivit: Human (tested or 100% immunogen sequence identity), Clonalit: IgG Polyclonal, Conjugation: Unconjugated. Also available conjugated with Biotin, FITC, HRP., Purificatio: Immunoaffinity purified, Modification: Unmodified, Immunoge: Synthetic peptide located between aa215-264 of human ACPP (P15309, NP_001090). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bovine, Bat, Rabbit, Guinea pig, Turkey, Zebra finch, Platypus, Lizard (100%); Horse, Opossum, Chicken (92%); Galago (85%); Elephant (84%)., Epitop: aa215-264, Specificit: Human ACPP, Application: IHC
IHC - Paraffin (10 µg/ml)
Western blot (0.2 - 1 µg/ml), Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500., Presentatio: PBS, 0.09% sodium azide, 2% sucrose, Storag: Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee., About ACPP / PA: Uniprot: P15309 NCBI: NM_001099 NP_001090.2

Cat: RW-52-5216-126