Rabbit anti‑Human CCL25 / TECK Polyclonal RW-C104396-50

Antibody: CCL25 / TECK Rabbit anti-Human Polyclonal Antibody, Application: WB, ELISA, Reactivity: Human, Format: Unconjugated, Unmodified, Other formats: Biotin, Descriptio: TECK antibody RW-C104396 is an unconjugated rabbit polyclonal antibody to human TECK (CCL25). Validated for ELISA and WB., Targe: Human CCL25 / TECK, Synonym: CCL25 | C-C motif chemokine 25 | Ck beta-15 | TECK | Thymus-expressed chemokine | Small-inducible cytokine A25 | TECKvar | Chemokine TECK | Ckb15 | SCYA25 | Thymus expressed chemokine, Hos: Rabbit, Reactivit: Human (tested or 100% immunogen sequence identity), Clonalit: Polyclonal, Conjugation: Unconjugated. Also available conjugated with Biotin., Purificatio: Immunoaffinity purified, Modification: Unmodified, Immunoge: E.coli derived recombinant Human TECK., Specificit: Human TECK, Application: Western blot (0.1 - 0.2 µg/ml)
ELISA (0.5 - 2 µg/ml), Usag: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human normal small bowel. The recommended concentration is 0.5 ug/ml-1.0ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hTECK (100 ng/ml), a concentration of 10-15 ug/ml of this antibody is required. ELISA: To detect hTECK by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TECK (RW-C104509) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTECK. Western Blot: To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTECK is 1.5-3 ng/lane, under either reducing or non-reducing conditions., Presentatio: Lyophilized from PBS, pH 7.2, Reconstitutio: Reconstitute with sterile water to 0.1-1.0 mg/ml., Storag: Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee., About CCL25 / TEC: Uniprot: O15444 NCBI: NM_005624 NP_005615.2

Cat: RW-52-66944-114